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Micro- and Nano-Transport of Biomolecules English

  • Author David Bakewell
  • ISBN 978-87-7681-513-4
  • 1 edition
  • 96 pages

Description

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Contents

Preface

1 Introduction

1.1 Motivation: biomolecules in scientifi c context

1.2 Length scale of transport

1.3 Biomolecule transport example: engineered microdevices

1.4 Structure of this e-book

2 Biomolecules and their electrical properties

2.1 Biomolecules in cells

2.2 Biomolecules: structure and function

2.2.1 Nucleic acids

2.2.2 Proteins

2.2.3 Carbohydrates

2.2.4 Lipids

2.3 Biomolecules: electrical properties

2.3.1 Polyelectrolytes

2.3.2 DNA can be modeled as wormlike chain

2.3.3 Biomolecules and bioparticles

2.3.4 Electrical double layer

2.3.5 Introduction to dielectric polarization

2.3.6 Polarisation parameters: a brief view

2.3.7 Measurement of biomolecule polarisation parameters

2.4 Concluding remarks

3 Moving biomolecules using electric fi elds

3.1 Electrophoresis

3.2 Dielectrophoresis (DEP)

3.2.1 Polarisation and DEP biomolecule transport

3.2.2 Maxwell-Wagner interfacial polarisation

3.2.3 Maxwell-Wagner interfacial polarisation for bioparticles

3.2.4 Maxwell-Wagner polarisation for DNA

3.2.5 Counterion fluctuation polarisation

3.2.6 Counterion fluctuation polarisation for bioparticles

3.2.7 Counterion fluctuation polarisation for DNA

3.2.8 Other polarisation mechanisms

3.3 Micro-environments for biomolecule transport

3.4 Concluding remarks

4 Basic micro- and nano-transport

4.1 Inertial, friction and sedimentation forces on single biomolecules

4.2 Electromagnetic forces acting on single biomolecules

4.2.1 Electric fields and electrophoresis

4.2.2 Inhomogenous electric fields and dielectrophoresis

4.2.3 Electroosmosis

4.2.4 Magnetic fields

4.3 Thermal fluctuations

4.4 Combining forces for predicting single bioparticle trajectory

4.5 Langevin equation for a single bioparticle (biomolecule)

4.6 Langevin equation stochastic integration and the modifi ed diffusion equation (MDE)

4.6.1 Example of one-dimensional (1D) MDE transport

4.6.3 3D MDE transport and parameters

4.7 Concluding remarks

5 Observing, quantifying and simulating electrically driven biomolecule microtransport

5.1 Micro-device and experimental arrangement

5.2 Observations and quantitative measurements

5.2.1 Using geometry of DEP force aids quantifi cation

5.2.2 DEP collections exhibit frequency and voltage dependence

5.3 Simulations of electrically driven biomolecule micro-transport

5.3.1 Determining the dielectrophoretic force throughout the chamber.

5.3.2 Solutions of the MDE for predicting bioparticle collections

5.4 Brief discussion of experiments and theory

5.5 Concluding remarks

6 References

6.1 General – selected books

6.2 Research articles and other reading

Preface

The micro- and nano- transport of biomolecules is of interest to a wide range of scientific and engineering communities. Application areas include miniaturized technology that will support and advance key sectors, including healthcare, food provisioning, environment services, etc. This ebook is generally intended for undergraduate students from chemical, life and physical sciences wanting to find out about the basic properties of biomolecules and how they can be transported in liquids on the micro- to nano-scale. The e-book tends to be oriented towards engineering aspects, especially with the transport of biomolecules in micro-devices powered electrically. It is hoped it will also be useful for interdisciplinary researchers surveying the field of biomolecule transport.

Much of the book can be read with no more than high school level of science and mathematics and selected areas that require engineering mathematics can be omitted if need be. Vector notation for example has been deliberately omitted until Chapter 4. At the same time the more mathematical sections in Chapter 4 are expected to be useful for researchers entering this area of science.

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